BTC 202 - Molecular Biology Laboratory
2 Lecture Hours 6 Lab Hours
Prerequisites: BTC 101 or BIO 207
This course introduces students to modern molecular biology theory and laboratory practices. Special topics will include advanced molecular biology methods used in protein and nucleic acid analysis, enzyme-mediated reactions, genetic transformations, DNA sequence interpretation and gene amplification.
Upon successful completion of the course, the student will:
- Contrast agarose and polyacrylamide gel electrophoresis protocols.
- Employ molecular techniques to isolate and characterize nucleic acids and proteins.
- Apply enzyme-linked immunosorbent assay techniques to biomedical problems.
- Interpret enzyme mediated biochemical reaction rates.
- Perform experiments to study restriction enzyme activity.
- Compare Southern blot analysis of DNA and Western blot analysis of proteins.
- Interpret DNA sequencing data
- Apply the polymerase chain reaction to amplify target DNA.
- Perform RNA analysis using reverse transcription polymerase chain reaction.
- Execute basic laboratory techniques used in genetic transformation of bacteria.
- DNA and protein isolation and separation using electrophoresis
- Enzyme rates of reactions
- Enzyme-linked immunosorbent assay (ELISA)
- Western blot analysis of protein
- Restriction enzyme analysis
- Polymerase chain reaction (PCR)
- DNA and RNA isolation and analysis
- Southern blot analysis of DNA
- DNA sequencing analysis
- Bacterial cell transformation
Textbooks/materials as required by instructor.
Students who successfully complete this course acquire general knowledge, skills and abilities that align with CCAC’s definition of an educated person. Specifically, this course fulfills these General Education Goals:
Approved By: Bullock, Quintin Date Approved: 02/27/2020
- Quantitative and Scientific Reasoning
- Technological Competence
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