Dec 03, 2023  
2020-2021 Catalog 
2020-2021 Catalog [ARCHIVED CATALOG]

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BTC 202 - Molecular Biology Laboratory

Credits: 4
2 Lecture Hours 6 Lab Hours

Prerequisites: BTC 101  or BIO 207  

This course introduces students to modern molecular biology theory and laboratory practices.  Special topics will include advanced molecular biology methods used in protein and nucleic acid analysis, enzyme-mediated reactions, genetic transformations, DNA sequence interpretation and gene amplification.

Learning Outcomes
Upon successful completion of the course, the student will:

  1. Contrast agarose and polyacrylamide gel electrophoresis protocols.
  2. Employ molecular techniques to isolate and characterize nucleic acids and proteins.
  3. Apply enzyme-linked immunosorbent assay techniques to biomedical problems.
  4. Interpret enzyme mediated biochemical reaction rates.
  5. Perform experiments to study restriction enzyme activity.
  6. Compare Southern blot analysis of DNA and Western blot analysis of proteins.
  7. Interpret DNA sequencing data
  8. Apply the polymerase chain reaction to amplify target DNA.
  9. Perform RNA analysis using reverse transcription polymerase chain reaction.
  10. Execute basic laboratory techniques used in genetic transformation of bacteria.
Listed Topics
  1. DNA and protein isolation and separation using electrophoresis
  2. Enzyme rates of reactions
  3. Enzyme-linked immunosorbent assay (ELISA)
  4. Western blot analysis of protein
  5. Restriction enzyme analysis
  6. Polymerase chain reaction (PCR)
  7. DNA and RNA isolation and analysis
  8. Southern blot analysis of DNA
  9. DNA sequencing analysis
  10. Bacterial cell transformation
Reference Materials
Textbooks/materials as required by instructor.
Students who successfully complete this course acquire general knowledge, skills and abilities that align with CCAC’s definition of an educated person. Specifically, this course fulfills these General Education Goals:
  • Communication
  • Quantitative and Scientific Reasoning
  • Technological Competence
Approved By: Bullock, Quintin Date Approved: 02/27/2020

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