BIO 156 - Molecular Genetics Laboratory
Credits: 2
4 Lab Hours
Prerequisites: BIO 151 OR BIO 207
Description
(Formerly BTC-202) This course introduces students to modern molecular genetics laboratory practices. Special topics include advanced molecular genetic methods used in nucleic acid analysis, genetic transformations, gene editing, DNA sequence interpretation and gene amplification.
Learning Outcomes
Upon successful completion of the course, the student will:
- Use agarose and polyacrylamide gel electrophoresis to separate nucleic acids.
- Utilize restriction enzymes to cut DNA for DNA profiling and genetic disease detection.
- Perform Southern blotting analysis of DNA.
- Interpret DNA sequencing data.
- Apply the polymerase chain reaction (PCR) to amplify target DNA.
- Apply gene editing and genetic transformation techniques to bacteria.
Listed Topics
- DNA extraction and separation
- Restriction enzyme analysis
- PCR
- Southern blot analysis of DNA
- DNA sequencing analysis
- Bacterial cell transformation
- Gene editing by use of clustered regularly interspaced short palindromic repeats (CRISPR)
Reference Materials
Textbooks/materials as required by instructor.
Students who successfully complete this course acquire general knowledge, skills and abilities that align with CCAC’s definition of an educated person. Specifically, this course fulfills these General Education Goals: - Communication
- Quantitative & Scientific Reasoning
- Technological Competence
Approved By: Dr. Quintin B. Bullock Date Approved: 3/13/2023
Last Reviewed: 3/13/2023
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