BTC 101 - Lab Techniques in Cellular Biology and Histology
Credits: 4
3 Lecture Hours 3 Lab Hours
Prerequisites: BIO 151 and MAT 108
Description
This course introduces the basic concepts and laboratory skills used in a biotechnology workplace. Laboratory skills emphasized include micropipetting, dilutions, preparing physiological solutions and spectroscopy. Modern and classic techniques used to determine cellular structure and enzymatic function will be explored. Students will become competent performing laboratory protocols including tissue embedding, staining, cell culture and immunohistochemistry.
Learning Outcomes
Upon successful completion of the course, the student will:
- Prepare common physiological solutions for laboratory use.
- Utilize precise measuring, sterile technique and light microscopy.
- Maintain a laboratory notebook and digital photo atlas.
- Demonstrate basic histological techniques.
- Develop skills to maintain cells in culture.
- Assemble analyzed experimental data.
Listed Topics
- Safety in the laboratory
- Laboratory measurements and solutions
- Laboratory notebooks and data collection
- Operation of scientific instruments
- Structural organization of the cell
- Staining techniques
- Immunofluorescence
Reference Materials
Textbooks/materials as required by instructor
Students who successfully complete this course acquire general knowledge, skills and abilities that align with CCAC’s definition of an educated person. Specifically, this course fulfills these General Education Goals: - Communication
- Critical Thinking and Problem Solving
- Technological Competence
Approved By: Bullock, Quintin Date Approved: 04/11/2019
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